84-3435-74　VEGF (MVCD1, Vascular Endothelial Growth Factor A, Vascular Endothelial Growth Factor, Vascular Permeability Factor, VEGF-A, VEGFA, VEGFA_HUMAN, VPF) 100ug　303696

特徴

• VEGF, a homodimeric glycoprotein of relative molecular mass 45,000, is the only mitogen that specifically acts on endothelial cells.
• It may be a major regulator of tumor angiogenesis in vivo.
• It is, however, structurally related to platelet-derived growth factor.
• VEGF shares homology with the PDGF A chain and B chain, including conservation of all 8 cysteines found in PDGFA and PDGFB.
• VEGF gene contains 8 exons.
• VEGF induces remodeling and enhances TH2-mediated sensitization and inflammation in the lung.
• And this gene also can regulate haematopoietic stem cell survival by an internal autocrine loop mechanism.
• It also stimulates neurogenesis in vitro and in vivo.
• Applications:Suitable for use in Western Blot and Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:Western Blot: 0.1-0.5ug/ml, the detection limit is ~0.25ng/lane under reducing conditions
• Immunohistochemistry (paraffin): 0.5-1ug/ml, boiling the paraffin sections in 10mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections
• Optimal dilutions to be determined by the researcher.
• Storage and Stability:Lyophilized powder may be stored at -20°C.
• Stable for 12 months after receipt at -20°C.
• Reconstitute with sterile ddH2O.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Reconstituted product is stable for 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.