84-3435-72　VCP, CT (15S Mg(2+) ATPase p97 Subunit, 15S Mg(2+)-ATPase p97 Subunit, ALS14, ATPase p97, IBMPFD, p97, TER ATPase, TERA, TERA_HUMAN, Transitional Endoplasmic Reticulum ATPase, Valosin Containing Protein, Valosin-containing Protein, Yeast Cdc48p Homolog) 100ug　303694

特徴

• Valosin-containing protein, also called CDC48, is an enzyme that in humans is encoded by the VCP gene.
• It is a member of the AAA+ (ATPase associated with various activities) protein family.
• The VCP gene maps to chromosome 9p13.3.
• It is necessary for the fragmentation of Golgi stacks during mitosis and for their reassembly after mitosis.
• It is involved in the formation of the transitional endoplasmic reticulum.
• This gene plays a role in vesicle transport and fusion, 26S proteasome function, and assembly of peroxisomes.
• It also involved in DNA damage response: recruited to double-strand breaks (DSBs) sites in a RNF8- and RNF168-dependent manner and promotes the recruitment of TP53BP1 at DNA damage sites.
• Applications:Suitable for use in Western Blot and Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:Western Blot: 0.1-0.5ug/ml, the detection limit is ~0.1ng/lane under reducing conditions
• Immunohistochemistry (frozen): 0.5-1ug/mlImmunohistochemistry (paraffin): 0.5-1ug/ml, boiling the paraffin sections in 10mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections
• Optimal dilutions to be determined by the researcher.
• Storage and Stability:Lyophilized powder may be stored at -20°C.
• Stable for 12 months after receipt at -20°C.
• Reconstitute with sterile ddH2O.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Reconstituted product is stable for 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.