SUMO-conjugating enzyme UBC9 (UBE2I), also called UBC9, is a protein that in humans is encoded by the UBE2I gene.
This gene encodes a member of the E2 ubiquitin-conjugating enzyme family.
It is mapped to 16p13.3.
UBC9 could fully complement the mutant phenotype of a yeast ubc9 mutant strain.
This gene may play a similar role via interaction with WT1, which is able to impose a block to cell cycle progression in eukaryotic cells.
It could support the growth of yeast ubc9 temperature-sensitive mutants at nonpermissive temperatures, indicating that the gene is a functional homolog of yeast ubc9.
UBC9 is specifically associated with FHIT, such as FHIT may be involved in cell cycle control through its interaction with UBC9.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 0.1-0.5ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile dH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from PBS, 5% BSA, 0.05% thimerosal, 0.05% sodium azide. Reconstitute with 200ul sterile dH2O.
Specificity：Recognizes human UBE2I. Species Crossreactivity: mouse and rat
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to aa108-122, IKQILLGIQELLNE, from human UBE2I at C-terminus, identical to the related mouse and rat sequences.
Shelf Life：1year
Swiss Prot Number：P63279
EU Commodity Code：30021010
商品名：UBE2I, CT (Homologous to Yeast UBC9, P18, SUMO 1 Conjugating Enzyme, SUMO 1 Protein Ligase, SUMO Conjugating Enzyme UBC9, SUMO Protein Ligase, SUMO-conjugating Enzyme UBC9, SUMO-protein Ligase, SUMO1 Conjugating Enzyme, SUMO1 Protein Ligase, UBC 9, UBC9, UBC9 Homolog, UBC9_HUMAN, UBCE 9, UBCE9, UBE 2I, Ube2i, Ubiquitin Carrier Protein 9, Ubiquitin Carrier Protein, Ubiquitin Carrier Protein I, Ubiquitin Conjugating Enzyme 9, Ubiquitin Conjugating Enzyme E2I (Homologous to Yeast UBC9), Ubiquitin Conjugating Enzyme E2I (UBC9 Homolog, Yeast), Ubiquitin Conjugating Enzyme E2I, Ubiquitin Conjugating Enzyme UbcE2A, Ubiquitin Like Protein SUMO 1 Conjugating Enzyme, Ubiquitin Like Protein SUMO1 Conjugating Enzyme, Ubiquitin Protein Ligase E2I, Ubiquitin Protein Ligase I, Ubiquitin-conjugating Enzyme E2 I, Ubiquitin-protein Ligase I) 100ug
