Lymphotoxin (previously known as tumor necrosis factor-beta) is a lymphokine cytokine.
It is a protein that is produced by Th1 type T-cells and induces vascular endothelial cells to change their surface adhesion molecules to allow phagocytic cells to bind to them.
Lymphotoxin is homologous to Tumor Necrosis Factor beta, but secreted by T-cells.
It is paracrine due to the small amounts produced.
The effects are similar to TNF-alpha, but TNF-beta is also important for the development of lymphoid organs.
Nedwin et al.
(1985) found that TNFA and TNFB are closely linked on chromosome 6.
Study of hybrid cells made with rearranged human chromosome 6 showed that both TNFA and TNFB map to the 6p23-q12 segment.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 0.1-0.5ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile dH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from PBS, 5% BSA, 0.05% thimerosal, 0.05% sodium azide. Reconstitute with 200ul sterile dH2O.
Specificity：Recognizes human TNF beta.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to aa45-59, AQTARQHPKMHLAHS, from human TNF beta at N-terminus.
