TIE2, also known as TEK tyrosine kinase, is mapped to 9p21.2.
This gene encodes a receptor that belongs to the protein tyrosine kinase Tie2 family.
The encoded protein possesses a unique extracellular region that contains two immunoglobulin-like domains, three epidermal growth factor (EGF)-like domains and three fibronectin type III repeats.
The ligand angiopoietin-1 binds to this receptor and mediates a signaling pathway that functions in embryonic vascular development.
Immunoblotting showed that TIE2 expression was increased by thyroid-stimulating hormone and agents that increased intracellular cAMP.
HSCs expressing the receptor tyrosine kinase TIE2 are quiescent and antiapoptotic and comprise a side population of HSCs that adhere to osteoblasts in the bone marrow niche.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 0.1-0.5ug/ml, detection limit is ~0.25ng/lane under reducing conditions
Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile dH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from PBS, 5% BSA, 0.05% sodium azide. Reconstitute with 200ul sterile dH2O.
Specificity：Recognizes human TIE2.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to human TIE2 expressed in E. coli. (Position: Q641-I830). Species sequence homology: mouse (91%)
Shelf Life：1year
Swiss Prot Number：Q02763
EU Commodity Code：30021010
商品名：TIE2 (Angiopoietin 1 Receptor, Angiopoietin-1 Receptor, CD202b, CD202b Antigen, CD202b Antigen, Endothelial Tyrosine Kinase, Endothelium Specific Receptor Tyrosine Kinase 2, hTIE 2, hTIE 2, hTIE2, hTIE2, Hyk, p140 TEK, p140 TEK, Soluble TIE2 Variant 1, Soluble TIE2 Variant 2, TEK, Tek Tyrosine Kinase, TEK Tyrosine Kinase Endothelial, Tek Tyrosine Kinase, Endothelial, TIE 2, TIE2_HUMAN, Tunica Interna Endothelial Cell Kinase, Tyrosine Kinase with Ig and EGF Homology Domains 2, Tyrosine Protein Kinase Receptor TEK, Tyrosine Protein Kinase Receptor TIE 2, Tyrosine Protein Kinase Receptor TIE 2, Tyrosine-protein Kinase Receptor TEK, Tyrosine-protein Kinase Receptor TIE-2, Venous Malformations Multiple Cutaneous and Mucosal, VMCM 1, VMCM, VMCM1) 100ug
