The protein encoded by this gene is a subunit of a disulfide-linked homotrimeric protein.
It is an adhesive glycoprotein that mediates cell-to-cell and cell-to-matrix interactions.
Also this protein can bind to fibrinogen, fibronectin, laminin, type V collagen and integrins alpha-V/beta-1.
This protein has been shown to play roles in platelet aggregation, angiogenesis, and tumorigenesis.
In addition, the thrombospondin-1 protein is a member of the thrombospondin family.
It is a multi-domain matrix glycoprotein that has been shown to be a natural inhibitor of neovascularization and tumorigenesis in healthy tissue.
Both positive and negative modulation of endothelial cell adhesion, motility, and growth have been attributed to TSP1.
This should not be surprising considering that TSP1 interacts with at least 12 cell adhesion receptors, including CD36, alphav integrins, beta1 integrins, syndecan, and integrin-associated protein (IAP or CD47).
It also interacts with numerous proteases involved in angiogenesis, including plasminogen, urokinase, matrix metalloproteinase, thrombin, cathepsin, and elastase.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 0.1-0.5ug/ml, the detection limit is ~0.1ng/lane under reducing conditions
Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months after receipt at -20°C.
Reconstitute with sterile ddH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from PBS, 5% BSA, 0.05% sodium azide. Reconstitute with 200ul sterile dH2O.
Specificity：Recognizes human THBS1.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to aa58-86, AFRIEDANLIPPVPDDKFQDLVDAVRAEK, from human THBS1 at N-terminus, different from the related mouse sequence by 5aa.
