The spine apparatus (SA) is a specialized form of endoplasmic reticulum (ER) that is found in a subpopulation of dendritic spines in central neurons.
The SA consists of a series of stacked discs that are thought to be connected to each other and to the dendritic system of ER-tubules.
The actin binding protein synaptopodin (which has originally been described in podocytes of the kidney) is an essential component of the SA.
Mice that lack the gene for synaptopodin do not form a spine apparatus.
The SA is believed to play a critical role in learning and memory.
In summary, an important function of the spine apparatus is the regulation of plasticity at individual synapses, a process known as metaplasticity.
The International Radiation Hybrid Mapping Consortium mapped the SYNPO gene to chromosome 5.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 0.1-0.5ug/ml, the detection limit is ~0.1ng/lane under reducing conditions
Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile dH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from PBS, 5% BSA, 0.05% sodium azide. Reconstitute with 200ul sterile dH2O.
Specificity：Recognizes human Synaptopodin. Species Crossreactivity: mouse
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo
Immunogen：Synthetic peptide corresponding to aa629-658, EKPKVTPNPDLLDLVQTADEKRRQRDQGEV, from human Synaptopodin at C-terminus, different from the related mouse sequence by 1aa, and identical to the related rat sequence.
