SYP (Synaptophysin) is located on the short arm of X chromosome (Xp11.23-p11.22).
The protein is a synaptic vesicle glycoprotein with four transmembrane domains weighing 38kD.
It is present in neuroendocrine cells and in virtually all neurons in the brain and spinal cord that participate in synaptic transmission.
It acts as a marker for neuroendocrine tumors, and its ubiquity at the synapse has led to the use of synaptophysin immunostaining for quantification of synapses.
Also, the protein binds cholesterol and is thought to direct targeting of vesicle- associated membrane protein 2 (synaptobrevin) to intracellular compartments.
Mutations in this gene are associated with X-linked mental retardation (XLMR).
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 0.1-0.5ug/ml, the detection limit is ~0.1ng/lane under reducing conditions
Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months after receipt at -20°C.
Reconstitute with sterile ddH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from PBS, 5% BSA, 0.05% sodium azide. Reconstitute with 200ul sterile dH2O.
Specificity：Recognizes human Synaptophysin. Species Crossreactivity: mouse and rat
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to aa1-26, MLLLADMDVVNQLVAGGQFRVVKEPL, from human Synaptophysin at N-terminus, identical to the related mouse and rat sequences.
