84-3435-29　Src (ASV, Avian Sarcoma Virus, C SRC, CSrc, Neuronal CSRC Tyrosine Specific Protein Kinase, Neuronal SRC, Oncogene SRC, P60 Src, P60-Src, P60Src, pp60c Src, pp60c-Src, pp60csrc, Proto Oncogene Tyrosine Protein Kinase Src, Proto-oncogene C-Src, Proto-oncogene Tyrosine-protein Kinase Src, Protooncogene SRC, Protooncogene SRC Rous Sarcoma, SRC Oncogene, SRC_HUMAN, SRC1, Tyrosine Kinase Pp60c Src, ...　303643

特徴

• Sarcoma (Scr) is a proto-oncogenic tyrosine kinase originally discovered by J.
• Michael Bishop and Harold E.
• Varmus.
• belongs to a family of non-receptor tyrosine kinases called Src family kinases.
• The human SRC protooncogene was assigned to chromosome 20.
• Its gene is mapped to 20q12-q13.
• The discovery of Src family proteins has been instrumental to the modern understanding of cancer as a disease where normally healthy cellular signalling has gone awry.
• This proto-oncogene may play a role in the regulation of embryonic development and cell growth.
• Applications:Suitable for use in Western Blot and Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:Western Blot: 0.1-0.5ug/ml, the detection limit is ~0.25ng/lane under reducing conditions
• Immunohistochemistry (paraffin): 0.5-1ug/ml, boiling the paraffin sections in 10mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections
• Optimal dilutions to be determined by the researcher.
• Storage and Stability:Lyophilized powder may be stored at -20°C.
• Stable for 12 months after receipt at -20°C.
• Reconstitute with sterile ddH2O.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Reconstituted product is stable for 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.