SFTPA1 encodes a lung surfactant protein that is a member of a subfamily of C-type lectins called collectins.
The encoded protein binds specific carbohydrate moieties found on lipids and on the surface of microorganisms.
This protein plays an essential role in surfactant homeostasis and in the defense against respiratory pathogens.
Mutations in this gene are associated with idiopathic pulmonary fibrosis.
Alternate splicing results in multiple transcript variants.
SFTPA2 is one of several genes encoding pulmonary-surfactant associated proteins (SFTPA) located on chromosome 10.
Mutations in this gene and a highly similar gene located nearby, which affect the highly conserved carbohydrate recognition domain, are associated with idiopathic pulmonary fibrosis.
The current version of the assembly displays only a single centromeric SFTPA gene pair rather than the two gene pairs shown in the previous assembly which were thought to have resulted from a duplication.
Applications:Suitable for use in Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 0.1-0.5ug/ml, the detection limit is ~0.1ng/lane under reducing conditions
Immunohistochemistry (frozen): 0.5-1ug/mlImmunohistochemistry (paraffin): 0.5-1ug/ml, boiling the paraffin sections in 10mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections
Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C.
Reconstitute with sterile ddH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from PBS, 5% BSA, 0.05% sodium azide. Reconstitute with 200ul sterile ddH2O.
Specificity：Recognizes human SFTPA1/2. Species Crossreactivity: mouse and rat
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to aa206-237, VNYTNWYRGEPAGRGKEQCVEMYTDGQWNDRN, from human SFTPA1/2 at C-terminus, different from the related mouse sequence by 4aa, and from the related rat sequence by 5aa.
Shelf Life：1year
Swiss Prot Number：Q8IWL1
EU Commodity Code：30021010
商品名：SFTPA1/2 CT (SP-A, 35kD Pulmonary Surfactant Associated Protein, 35kD Pulmonary Surfactant-associated Protein, Alveolar Proteinosis Protein, COLEC4, Collectin 4, Collectin-4, PSAP, PSP A, PSP-A, PSPA, Pulmonary Surfactant-associated Protein, 35kD, Pulmonary Surfactant Apoprotein PSP-A, Pulmonary Surfactant Associated Protein A1, Pulmonary Surfactant-associated Protein A1, SFTA1_HUMAN, SFTP1, SFTPA1, SFTPA1B, SP A, SP A1, SP-A, SP-A1, SPA, SPA1, Surfactant Protein A1, Surfactant Protein A1 Variant AB'D' 6A2, Surfactant Protein A1 Variant AB'D' 6A3, Surfactant Protein A1 Variant AB'D' 6A4, Surfactant Protein A1 Variant ACD' 6A2, Surfactant Protein A1 Variant ACD' 6A3, Surfactant Protein A1 Variant ACD' 6A4, Surfactant Protein A1 Variant AD' 6A, Surfactant Protein A1 Variant AD' 6A2, Surfactant Protein A1 Variant AD' 6A3, Surfactant Protein A1 Variant AD' 6A4, Surfactant Protein A1B, Surfactant, Pulmonary Associated Protein A1A, Surfactant, Pulmonary Associated Protein A1B, Surfactant-associated Protein, Pulmonary 1) 100ug
