SMAD4 (Mothers Against Decapentaplegic Drosophila Homolog of 4), also known as MADH4 or DPC4, is a protein that in humans is encoded by the SMAD4 gene.
It belongs to the Darfwin family of proteins that modulate members of the TGFbeta protein superfamily.
Hahn et al.
(1996) identified the SMAD4 gene on chromosome 18q21.1.
Howe et al.
(1998) identified the SMAD4 gene within a region on 18q21.1 defined by linkage analysis in kindred with juvenile polyposis syndrome.
To test directly the hypothesis that the SMAD4 gene is a tumor suppressor that is critical for transmitting signals from transforming growth factor-beta and related ligands.
SMAD4 plays a pivotal role in signal transduction of the transforming growth factor beta superfamily cytokines by mediating transcriptional activation of target genes.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 0.1-0.5ug/ml, the detection limit is ~0.1ng/lane under reducing conditions
Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months after receipt at -20°C.
Reconstitute with sterile ddH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from PBS, 5% BSA, 0.05% sodium azide. Reconstitute with 200ul sterile dH2O.
Specificity：Recognizes human Smad4. Species Crossreactivity: mouse and rat
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to aa526-552, EIHLHRALQLLDEVLHTMPIADPQPLD, from human Smad4 at C-terminus, identical to the related mouse and rat sequences.
Shelf Life：1year
Swiss Prot Number：Q13485
EU Commodity Code：30021010
商品名：Smad4, CT ((Small) Mothers Against Decapentaplegic, Deleted in Pancreatic Carcinoma, Deleted in Pancreatic Carcinoma Locus 4, Deletion Target in Pancreatic Carcinoma 4, DPC 4, DPC4, HSMAD4, JIP, MAD Homolog 4, MAD Mothers Against Decapentaplegic Drosophila Homolog 4, MAD Mothers Against Decapentaplegic Homolog 4, MADH 4, MADH4, Med, Medea, Mothers Against Decapentaplegic Homolog 4, Mothers Against DPP Homolog 4, SMAD 4, SMAD Family Member 4, SMAD Mothers Against DPP Homolog 4, SMAD4_HUMAN) 100ug
