NHE1, also known as SLC9A1, is an isoform of sodium–hydrogen antiporter that in humans is encoded by the SLC9A1 gene.
It is mapped to 1p36.11.
NHE1 is a ubiquitous membrane-bound enzyme involved in pH regulation of vertebrate cells.
It is specifically inhibited by the diuretic drug amiloride and activated by a variety of signals including growth factors, mitogens, neurotransmitters, tumor promoters, and others.
The plasma membrane ion exchanger NHE1 acts as an anchor for actin filaments to control the integrity of the cortical cytoskeleton.
And a structural role for NHE1 in regulating the cortical cytoskeleton that is independent of its function as an ion exchanger.
NHE1 is a plausible candidate gene for human essential hypertension.
Applications:Suitable for use in Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 0.1-0.5ug/ml, the detection limit is ~0.25ng/lane under reducing conditions
Immunohistochemistry (paraffin): 0.5-1ug/ml, boiling the paraffin sections in 10mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections
Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months after receipt at -20°C.
Reconstitute with sterile ddH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from PBS, 5% BSA, 0.05% sodium azide. Reconstitute with 200ul sterile dH2O.
Specificity：Recognizes human SLC9A1. Species Crossreactivity: mouse and rat
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Recombinant protein corresponding to human SLC9A1 expressed in E. coli. (Position: H543-Q815). Species sequence homology: mouse (92%) and rat (93%)
Shelf Life：1year
Swiss Prot Number：P19634
EU Commodity Code：30021010
商品名：SLC9A1 (Amiloride-sensitive, APNH, APNH1, Na Li Countertransporter, Na(+)/H(+) Antiporter, Na(+)/H(+) Exchanger 1, Na+ H+ Antiporter Amiloride-sensitive, Na+ H+ Antiporter, Na+ H+ Exchanger 1, NHE-1, NHE1, SL9A1_HUMAN, SLC9A1, Sodium Hydrogen Exchanger 1, Sodium/Hydrogen Exchanger 1, Solute Carrier Family 9, Solute Carrier Family 9 Member 1, Solute Carrier Family 9 Sodium Hydrogen Exchanger Isoform 1 Antiporter Na+ H+ Amiloride Sensitive, Solute Carrier Family 9 Subfamily A (NHE1 Cation Proton Antiporter 1) Member 1, Solute Carrier Family 9 Subfamily A Member 1) 100ug
