SLC6A4 (Solute carrier family 6, member 4), also known as SERT (serotonin transporter), is a monoamine transporter protein.
The SLC6A4 gene spans 31kb and contains 14 exons.
This protein integral membrane protein transports the neurotransmitter serotonin from synaptic spaces into presynaptic neurons.
This transport of serotonin by the SERT protein terminates the action of serotonin and recycles it in a sodium-dependent manner.
This protein is the target of many antidepressant medications, including those of the SSRI class.
It is a member of the sodium: neurotransmitter symporter family.
A repeat length polymorphism in the promoter of this gene has been shown to affect the rate of serotonin uptake and may play a role in sudden infant death syndrome, aggressive behavior in Alzheimer disease patients, post-traumatic stress disorder and depression-susceptibility in people experiencing emotional trauma.
Applications:Suitable for use in Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 0.1-0.5ug/ml, the detection limit is ~0.1ng/lane under reducing conditions
Immunohistochemistry (paraffin): 0.5-1ug/ml, boiling the paraffin sections in 10mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections
Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months after receipt at -20°C.
Reconstitute with sterile ddH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from PBS, 5% BSA, 0.05% sodium azide. Reconstitute with 200ul sterile dH2O.
Specificity：Recognizes human SLC6A4. Species Crossreactivity: rat
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Rt
Immunogen：Synthetic peptide corresponding to aa57-85, DDTRHSIPATTTTLVAELHQGERETWGKK, from human SLC6A4 at N-terminus, different from the related mouse and rat sequences by 6aa.
Shelf Life：1year
Swiss Prot Number：P31645
EU Commodity Code：30021010
商品名：SLC6A4, NT (5 HTT, 5 HTTLPR, 5 Hydroxytryptamine (Serotonin) Transporter, 5 Hydroxytryptamine Transporter, 5HT Transporter, 5HTT, hSERT, HTT, Na+/Cl- Dependent Serotonin Transporter, OCD1, SC6A4_HUMAN, Serotonin Transporter 1, SERT, SERT1, SLC6A4, Sodium Dependent Serotonin Transporter, Sodium-dependent Serotonin Transporter, Solute Carrier Family 6 (Neurotransmitter Transporter) Member 4, Solute Carrier Family 6 (Neurotransmitter Transporter, Serotonin), Member 4, Solute Carrier Family 6 Member 4) 100ug
