84-3434-98　SIRT6 (Mono ADP Ribosyltransferase Sirtuin 6, Mono-ADP-ribosyltransferase Sirtuin-6, NAD-dependent Deacetylase Sirtuin-6, NAD-dependent Protein Deacetylase Sirtuin-6, Regulatory Protein SIR2 Homolog, SIR2 Like 6, SIR2 Like Protein 6, Sir2 Related Protein Type 6, SIR2-like Protein 6, SIR2L6, SIRT 6, SIRT6_HUMAN, Sirtuin (Silent Mating Type Information Regulation 2 Homolog) 6 (S. cerevisiae), Si ...　303604

特徴

• SIRT6 is also known as SIR2L6.
• This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein.
• Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes.
• The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA.
• Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity.
• The protein encoded by this gene is included in class IV of the sirtuin family.
• Alternative splicing results in multiple transcript variants.
• Applications:Suitable for use in Western Blot and Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:Western Blot: 0.1-0.5ug/ml, the detection limit is ~0.1ng/lane under reducing conditions
• Immunohistochemistry (paraffin): 0.5-1ug/ml, boiling the paraffin sections in 10mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections
• Optimal dilutions to be determined by the researcher.
• Storage and Stability:Lyophilized powder may be stored at -20°C.
• Stable for 12 months after receipt at -20°C.
• Reconstitute with sterile ddH2O.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Reconstituted product is stable for 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.