Sirtuin 1, also known as SIR2L1 or SIRT1, is a protein that in humans is encoded by the SIRT1 gene.
It is mapped to 10q21.3.
Sirtuin 1 is a member of the sirtuin family of proteins, homologs of the Sir2 gene in S.
cerevisiae.
Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes.
Sirtuin 1 is downregulated in cells that have high insulin resistance and inducing its expression increases insulin sensitivity, suggesting the molecule is associated with improving insulin sensitivity.
Furthermore, Sirtuin 1 was shown to de-acetylate and affect the activity of both members of the PGC1-alpha/ERR-alpha complex, which are essential metabolic regulatory transcription factors.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 0.1-0.5ug/ml, the detection limit is ~0.25ng/lane under reducing conditions
Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months after receipt at -20°C.
Reconstitute with sterile ddH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from PBS, 5% BSA, 0.05% sodium azide. Reconstitute with 200ul sterile dH2O.
Specificity：Recognizes human SIRT1.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to human SIRT1 expressed in E. coli. (Position: R112-K311). Species sequence homology: mouse (90%)
Shelf Life：1year
Swiss Prot Number：Q96EB6
EU Commodity Code：30021010
商品名：SIRT1 (75SirT1, HSIR2, HSIRT1, HST2, S. cerevisiae, Homolog of, NAD Dependent Deacetylase SIRT1, NAD Dependent Deacetylase Sirtuin 1, NAD Dependent Protein Deacetylase Sirtuin 1, Regulatory Protein SIR2 Homolog 1, SIR1_HUMAN, SIR2 Like 1, SIR2 Like Protein 1, SIR2, S.cerevisiae, Homolog-like 1, SIR2-like Protein 1, SIR2Alpha, SIR2alpha Protein, SIR2L1, SIRT 1, Sirt1, SIRT1 Sir2 Like Proteins (Siruitins) Type 1, SIRT1: Sirtuin (Silent Mating Type Information Regulation 2 Homolog) 1 (S. cerevisiae), SirtT1 75kD Fragment, Sirtuin (Silent Mating Type Information Regulation 2 Homolog) 1 (S. cerevisiae), Sirtuin 1, Sirtuin, Sirtuin Type 1) 100ug
