Sex hormone-binding globulin (SHBG) is a glycoprotein that binds to the sex hormones, androgen and estrogen.
SHBG is produced mostly by the liver and is released into the bloodstream.
Other sites that produce SHBG include the brain, uterus, testes, and placenta.
SHBG has both enhancing and inhibiting hormonal influences.
It decreases with high levels of insulin, growth hormone, insulin-like growth factor 1 (IGF-1), androgens, prolactin and transcortin.
High estrogen, andthyroxine cause it to increase.
It has been found that the monosaccharides glucose and fructose reduced human SHBG production by hepatocytes, irrespective of the presence of insulin, by downregulation of hepatocyte nuclear factor-4A and replacement of HNF4A by COUP-TFI at a cis-element within the human SHBG promoter, coincident with repression of its transcriptional activity.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 0.1-0.5ug/ml, the detection limit is ~0.1ng/lane under reducing conditions
Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months after receipt at -20°C.
Reconstitute with sterile ddH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from PBS, 5% BSA, 0.05% sodium azide. Reconstitute with 200ul sterile dH2O.
Specificity：Recognizes human SHBG.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to human SHBG expressed in E. coli. (Position: L30-A210). Species sequence homology: mouse and rat (72%)
