84-3434-85　SerpinB2 (Monocyte Arg Serpin, Monocyte Arg-serpin, Monocyte Arginine-serpin, Monocyte-derived Plasminogen Activator Inhibitor, PAI, PAI-2, PAI2, PAI2_HUMAN, Placental Plasminogen Activator Inhibitor, PLANH2, Plasminogen Activator Inhibitor 2, Plasminogen Activator Inhibitor, Type 2, Plasminogen Activator Inhibitor, Type II (Arginine-serpin), Serine (or Cysteine) Proteinase Inhibitor, Clade B ...　303590

特徴

• SERPINB2 is also known as PAI or PAI2.
• Plasminogen activator inhibitor-2 (placental PAI) is acoagulation factor that inactivates tPA and urokinase.
• It is present in most cells, especially monocytes/macrophages.
• PAI-2 exists in two forms, a 60kD extracellular glycosylated form and a 43kD intracellular form.
• It is present only at detectable quantities in blood during pregnancy, as it is produced by the placenta, and may explain partially the increased rate of thrombosis during pregnancy.
• The majority of expressed PAI-2 remains unsecreted due to the presence of an inefficient internal signal peptide.
• Applications:Suitable for use in Western Blot and Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:Western Blot: 0.1-0.5ug/ml, the detection limit is ~0.1ng/lane under reducing conditions
• Immunohistochemistry (paraffin): 0.5-1ug/ml, boiling the paraffin sections in 10mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections
• Optimal dilutions to be determined by the researcher.
• Storage and Stability:Lyophilized powder may be stored at -20°C.
• Stable for 12 months after receipt at -20°C.
• Reconstitute with sterile ddH2O.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Reconstituted product is stable for 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.