Securin, also known as pituitary tumor-transforming 1 or EAP1 is a protein that in humans is encoded by the PTTG1 gene.
PTTG1 gene is mapped to 5q33.3.
The encoded protein is a homolog of yeast securin proteins, which prevent separins from promoting sister chromatid separation.
It is an anaphase-promoting complex(APC) substrate that associates with a separin until activation of the APC.
The gene product has transforming activity in vitro and tumorigenic activity in vivo, and the gene is highly expressed in various tumors.
The gene product contains 2 PXXP motifs, which are required for its transforming and tumorigenic activities, as well as for its stimulation of basic fibroblast growth factor expression.
It also contains a destruction box(D box) that is required for its degradation by the APC.
The acidic C-terminal region of the encoded protein can act as a transactivation domain.
The gene product is mainly a cytosolic protein, although it partially localizes in the nucleus.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 0.1-0.5ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile dH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from PBS, 5% BSA, 0.05% thimerosal, 0.05% sodium azide. Reconstitute with 200ul sterile dH2O.
Specificity：Recognizes human Securin.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to aa146-161, LMILDEERELEKLFQL, from human Securin at C-terminus.
