84-3434-81　SDHA (MD1GG, DHSA_HUMAN, Flavoprotein Subunit of Complex II, FP, PGL5, SDH 2, SDH1, SDH2, SDHF, Succinate Dehydrogenase [Ubiquinone] Flavoprotein Subunit, Succinate Dehydrogenase [Ubiquinone] Flavoprotein Subunit Mitochondrial, Succinate Dehydrogenase [Ubiquinone] Flavoprotein Subunit, Mitochondrial, Succinate Dehydrogenase Complex Flavoprotein Subunit, Succinate Dehydrogenase Complex Flavopro ...　303586

特徴

• Complex II of the mitochondrial respiratory chain, also known as succinate dehydrogenase or succinate:ubiquinone oxidoreductase, consists of 4 nuclear-encoded polypeptides, these are the flavoprotein subunit (SDHA), the iron sulfur protein subunit (SDHB), and the integral membrane protein subunits SDHC and SDHD.
• SDHA is an acronym for succinate dehydrogenase complex subunit A.
• The succinate dehydrogenase (SDH) protein complex catalyzes the oxidation of succinate (succinate + ubiquinone => fumarate + ubiquinol).
• The SDHA subunit is connected to the SDHB subunit on the hydrophilic, catalytic end of the complex, and weighs 72.7kD.
• Mutations in the SDHA subunit have a distinct pathology from mutations in the SDHB/SDHC/SDHD subunits; it is the only subunit to never have shown tumor suppressor behavior.
• Heterozygous carriers of an SDHA mutation do not develop paragangliomas as has been seen for mutations in the other subunits.
• This appears to be due to the expression of two similar SDHA genes (Types I and II) in the paraganglia system.
• Applications:Suitable for use in Western Blot.
• Other applications not tested.
• Recommended Dilution:Western Blot: 0.1-0.5ug/mlOptimal dilutions to be determined by the researcher.
• Storage and Stability:Lyophilized powder may be stored at -20°C.
• Stable for 12 months after receipt at -20°C.
• Reconstitute with sterile ddH2O.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Reconstituted product is stable for 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.