Runt-related transcription factor 3, also called AML2, is a protein that in humans is encoded by the RUNX3 gene.
The RUNX3 gene encodes a Runt-related transcription factor, which is part of the RUNX gene family.
By fluorescence in situ hybridization, RUNX3 was assigned to human chromosome 1p36.11.
RUNX3 binds to the core site of murine Leukemia virus, the core sequences in the enhancer of the polyomavirus, and also to the enhancers of the T-cell receptor genes.
It may be involved in the control of cellular proliferation and/or differentiation.
Applications:Suitable for use in Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 0.1-0.5ug/mlImmunohistochemistry (paraffin): 0.5-1ug/ml, boiling the paraffin sections in 10mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections
Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months after receipt at -20°C.
Reconstitute with sterile ddH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from PBS, 5% BSA, 0.05% sodium azide. Reconstitute with 200ul sterile dH2O.
Specificity：Recognizes human RUNX3. Species Crossreactivity: mouse and rat
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Recombinant protein corresponding to human RUNX3 expressed in E. coli. (Position: M128-Y270). Species sequence homology: mouse (93%)
Shelf Life：1year
Swiss Prot Number：Q13761
EU Commodity Code：30021010
商品名：RUNX3 (Acute Myeloid Leukemia 2 Protein, Acute Myeloid Leukemia Gene 2, AML 2, AML2, CBF alpha 3, CBF-alpha-3, CBFA 3, CBFA3, Core Binding Factor alpha 3 Subunit, Core Binding Factor, Core Binding Factor Runt Domain alpha Subunit 3, Core Binding Factor Subunit alpha 3, Core-binding Factor, Core-binding Factor Subunit alpha-3, Oncogene AML 2, Oncogene AML-2, PEA2 alpha C, PEA2-alpha C, PEBP2 alpha C, PEBP2-alpha C, Pebp2a3, PEBP2aC, Polyomavirus Enhancer Binding Protein 2 alpha C Subunit, Polyomavirus Enhancer-binding Protein 2 alpha C Subunit, Runt Domain alpha Subunit 3, Runt Related Transcription Factor 3, Runt-related Transcription Factor 3, RUNX 3, Runx3, SL3 3 Enhancer Factor 1 alpha C Subunit, SL3-3 Enhancer Factor 1 alpha C Subunit, SL3/AKV Core Binding Factor alpha C Subunit, SL3/AKV Core-binding Factor alpha C Subunit, Transcription Factor AML2) 100ug
