84-3434-74　RIPK1 (RIP, Cell Death Protein RIP, Receptor (TNFRSF) Interacting Serine Threonine Kinase 1, Receptor Interacting Protein 1, Receptor Interacting Protein, Receptor Interacting Serine Threonine Protein Kinase 1, Receptor TNFRSF Interacting Serine Threonine Kinase 1, Receptor-interacting Protein 1, Receptor-interacting Serine/Threonine-protein Kinase 1, Rinp, RIP 1, Rip-1, RIP1, RIPK 1, Ripk1, R ...　303578

特徴

• RIPK1, also known as RIP or RIP1, is an enzyme that in humans is encoded by the RIPK1 gene.
• It is mapped to 6p25.2.
• RIPK1 is a key signaling molecule in the programmed necrosis pathway, which plays important roles in development, tissue damage response, and antiviral immunity.
• RIPK1 is known to have function in a variety of cellular pathways including the NF-kB pathway and programmed necrotic cell death (necroptosis).
• The kinase domain, while important for necroptotic (programmed necrotic) functions, it appears dispensable for other lethal, as well as pro-survival roles.
• Also, proteolytic processing of RIPk1, through both caspase-dependent and independent mechanisms, triggers lethality that is dependent on the generation of one or more specific C-terminal cleavage product(s) of RIPk1 upon stress.
• Applications:Suitable for use in Western Blot.
• Other applications not tested.
• Recommended Dilution:Western Blot: 0.1-0.5ug/ml, the detection limit is ~0.25ng/lane under reducing conditions
• Optimal dilutions to be determined by the researcher.
• Storage and Stability:Lyophilized powder may be stored at -20°C.
• Stable for 12 months after receipt at -20°C.
• Reconstitute with sterile ddH2O.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Reconstituted product is stable for 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.