Resistin, also known as adipose tissue-specific secretory factor (ADSF) or C/EBP-epsilon- regulated myeloid-specific secreted cysteine-rich protein (XCP1) is a cysteine-rich protein that in humans is encoded by the RETN gene.
The resistin gene comprises 4 exons, the first of which is untranslated, and spans ~1,750bp.
The human resistin gene is localized to a cloned fragment of human chromosome 19.
In primates, pigs and dogs, resistin is secreted by immune and epithelial cells while in rodents, it is secreted by adipose tissue.
Resistin is a cytokine whose physiologic role has been the subject of much controversy regarding its involvement with obesity and type II diabetes mellitus.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:ELISA: 0.1-0.5ug/mlWestern Blot: 0.1-0.5ug/ml, the detection limit is ~0.25ng/lane under reducing conditions
Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months after receipt at -20°C.
Reconstitute with sterile ddH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size:100ug
Host:rabbit
Source Antibody:human
Grade:Affinity Purified
Purity:Purified by immunoaffinity chromatography.
Form:Supplied as a lyophilized powder from PBS, 5% BSA, 0.05% sodium azide. Reconstitute with 200ul sterile dH2O.
Specificity:Recognizes human Resistin.
Isotype:IgG
Calc Applications Abbrev:E WB
Calc Crossreactivity:Hu
Immunogen:Recombinant protein corresponding to human Resistin expressed in E. coli. (Position: K19-P108). Species sequence homology: mouse (59%)
Shelf Life:1year
Swiss Prot Number:Q9HD89
EU Commodity Code:30021010
商品名:Resistin (Adipose Tissue Specific Secretory Factor, Adipose Tissue-specific Secretory Factor, ADSF, C/EBP epsilon Regulated Myeloid Specific Secreted Cysteine Rich Protein, C/EBP epsilon Regulated Myeloid Specific Secreted Cysteine Rich Protein Precursor 1, C/EBP-epsilon-regulated Myeloid-specific Secreted Cysteine-rich Protein, Cysteine Rich Secreted Protein A12 alpha Like 2, Cysteine Rich Secreted Protein FIZZ3, Cysteine-rich Secreted Protein A12-alpha-like 2, Cysteine-rich Secreted Protein FIZZ3, Dri, FIZZ 3, FIZZ3, Found in Inflammatory Zone 3, HXCP 1, HXCP1, Resistin delta2, RETN 1, RETN, RETN_HUMAN, RETN1, RSTN, XCP 1, XCP1) 100ug