84-3434-67　Rad51 (BRCA1/BRCA2 Containing Complex, Subunit 5, BRCC 5, BRCC5, DNA Repair Protein RAD51 Homolog 1, DNA Repair Protein Rhp51, E coli RecA Homolog, Homolog of E coli RecA, Homolog of S cerevisiae RAD51, HRAD51, HsRad51, MRMV2, Rad 51, RAD51, RAD51 Homolog (RecA Homolog, E. coli) (S. cerevisiae), RAD51 Homolog A, RAD51 Homolog, RAD51 Homolog S. cerevisiae, RAD51 Recombinase, RAD51 S cerevisiae ...　303571

特徴

• DNA repair protein RAD51 homolog 1, also known as RAD51A, is a human gene.
• The Rad51 gene, HsRAD51, is a homolog of RecA of Escherichia coli and functions in recombination and DNA repair.
• BRCA1 and BRCA2 proteins form a complex with Rad51, and these genes are thought to participate in a common DNA damage response pathway associated with the activation of homologous recombination and double-strand break repair.
• RAD51 is also found to interact with BRCA1 and BRCA2, which may be important for the cellular response to DNA damage.
• BRCA2 is shown to regulate both the intracellular localization and DNA-binding ability of this protein.
• Loss of these controls following BRCA2 inactivation may be a key event leading to genomic instability and tumorigenesis.
• Applications:Suitable for use in Western Blot and Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:Western Blot: 0.1-0.5ug/ml, the detection limit is ~0.1ng/lane under reducing conditions
• Immunohistochemistry (paraffin): 0.5-1ug/ml, boiling the paraffin sections in 10mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections
• Optimal dilutions to be determined by the researcher.
• Storage and Stability:Lyophilized powder may be stored at -20°C.
• Stable for 12 months after receipt at -20°C.
• Reconstitute with sterile ddH2O.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Reconstituted product is stable for 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.