84-3434-51　Progesterone Receptor (NR3C3, Nuclear Receptor Subfamily 3 Group C Member 3, PGR, PR, PRA, PRB, PRGR_HUMAN, Progestin Receptor Form A, Progestin Receptor Form B) 100ug　303554

特徴

• The progesterone receptor (PR) is an intracellular steroid receptor that specially binds progesterone in humans.
• PR has been a member of the steroid receptor superfamily.
• It is encoded by a single PGR gene residing on chromosome 11q22.
• The PGR gene uses separate promoters and translational start sites to produce 2 isoforms, PRA and PRB, which are identical except for an additional 165aa present only in the N terminus of PRB.
• It can be observed in human breast tissues.
• The proteins function as dimeric molecules in nuclei to regulate the transcription of target genes in a ligand-responsive manner.
• Applications:Suitable for use in Western Blot and Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:Western Blot: 0.1-0.5ug/mlImmunohistochemistry (paraffin): 0.5-1ug/ml, boiling the paraffin sections in 10mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections
• Optimal dilutions to be determined by the researcher.
• Storage and Stability:Lyophilized powder may be stored at -20°C.
• Stable for 12 months after receipt at -20°C.
• Reconstitute with sterile ddH2O.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Reconstituted product is stable for 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.