84-3434-49　PP2A-alpha (PP2A alpha, PP2AA_HUMAN, PP2Aalpha, PP2Ac, PP2CA, PP2Calpha, PPP2CA, Protein Phosphatase 2 (Formerly 2A) Catalytic Subunit alpha Isoform, Protein Phosphatase 2 Catalytic Subunit alpha Isoform, Protein Phosphatase 2, Catalytic Subunit, alpha Isozyme, Protein Phosphatase 2A Catalytic Subunit, alpha Isoform, Replication Protein C, RP C, RP-C, RPC, Serine/Threonine Protein Phosphatase ...　303552

特徴

• The catalytic subunit of human protein phosphatase 2A (PPP2CA) encodes a 309aa polypeptide.
• It is localized to chromosome 5.
• The gene (~30kbp) is composed of seven exons and six introns.
• It is predicted to be important for phosphatase enzymatic activity.
• Methylation of the C-terminal leucine residue (Leu309) of protein serine/threonine phosphatase 2A catalytic subunit (PP2AC) is known to regulate catalytic activity in vitro.
• Furthermore, PP2A has a fundamental role in cardiac function, and suggests that disturbances in protein phosphatase expression and activity may cause or exacerbate the course of cardiac diseases.
• Applications:Suitable for use in Western Blot and Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:Western Blot: 0.1-0.5ug/ml, the detection limit is ~0.1ng/lane under reducing conditions
• Immunohistochemistry (paraffin): 0.5-1ug/ml, boiling the paraffin sections in 10mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections
• Optimal dilutions to be determined by the researcher.
• Storage and Stability:Lyophilized powder may be stored at -20°C.
• Stable for 12 months after receipt at -20°C.
• Reconstitute with sterile ddH2O.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Reconstituted product is stable for 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.