84-3434-37　PKC epsilon (KPCE_HUMAN, NPKC epsilon, NPKC-epsilon, PKCE, Pkcea, PRKCE, Protein Kinase C epsilon, Protein Kinase C epsilon Type) 100ug　303537

特徴

• Protein kinase C epsilon type, also known as PKCE, is an enzyme that in humans is encoded by the PRKCE gene.
• The protein encoded by this gene is one of the PKC family members.
• PRKCE is mapped to 2p21.
• This kinase has been shown to be involved in many different cellular functions, such as apoptosis, cardio protection from ischemia, heat shock response, as well as insulin exocytosis.
• It has been found that activation of PRKCE can induce VR1 channel activity at room temperature in the absence of any other agonist.
• PRKCE gene plays a role in apoptosis signaling pathways in thyroid cells and it has been indicated that a naturally occurring PRKCE mutant that functions as a dominant negative can block cell death triggered by a variety of stimuli.
• Expression of PRKCE inhibits chemotherapy-induced caspase-3 activation and apoptosis, thereby leading to cell survival.
• Applications:Suitable for use in Western Blot and Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:Western Blot: 0.1-0.5ug/ml, the detection limit is ~0.1ng/lane under reducing conditions
• Immunohistochemistry (paraffin): 0.5-1ug/ml, boiling the paraffin sections in 10mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections
• Optimal dilutions to be determined by the researcher.
• Storage and Stability:Lyophilized powder may be stored at -20°C.
• Stable for 12 months after receipt at -20°C.
• Reconstitute with sterile ddH2O.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Reconstituted product is stable for 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.