Peptidyl-prolyl cis-trans isomerase NIMA-interacting 1, also called DOD, is an enzyme that in humans is encoded by the PIN1 gene.
It is mapped to 19p13.2.
The enzyme binds to a subset of proteins and thus plays a role as a post phospho PIN1rol in regulating protein function.
Studies have shown that the deregulation of PIN1 may play a pivotal role in various diseases.
Notably, the up-regulation of PIN1 may be implicated in certain cancers, and the down-regulation of Pin1 may be implicated in Alzheimer's disease.
Inhibitors of PIN1 may have therapeutic implications for cancer and immune disorders.
PIN1 activity regulates the outcome of proline-directed kinase (e.g.
MAPK, CDK or GSK3) signalling and consequently regulates cell proliferation (in part through control of cyclin D1 levels and stability) and cell survival.
PIN1 also has an essential role in maintaining cell proliferation and regulating cyclin D1 function.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 0.1-0.5ug/ml, the detection limit is ~0.1ng/lane under reducing conditions
Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months after receipt at -20°C.
Reconstitute with sterile ddH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from PBS, 5% BSA, 0.05% sodium azide. Reconstitute with 200ul sterile dH2O.
Specificity：Recognizes human Pin1. Species Crossreactivity: mouse and rat
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Recombinant protein corresponding to human Pin1 expressed in E. coli. (Position: M1-E163). Species sequence homology: mouse (95%)
Shelf Life：1year
Swiss Prot Number：Q13526
EU Commodity Code：30021010
商品名：Pin1 (DOD, DODO, Drosophila, Homolog of, NIMA Interacting 1, Peptidyl Prolyl Cis Trans Isomerase NIMA Interacting 1, Peptidyl Prolyl Cis/Trans Isomerase NIMA Interacting, Peptidyl-prolyl Cis-trans Isomerase NIMA-interacting 1, Peptidyl-prolyl Cis-trans Isomerase Pin1, Peptidylprolyl Cis/Trans Isomerase NIMA Interacting 1, Pin 1, PIN1_HUMAN, PPIase Pin1, Prolyl Isomerase, Protein (Peptidylprolyl Cis/Trans Isomerase) NIMA Interacting 1, Protein NIMA Interacting 1, Rotamase Pin1, UBL 5, UBL5) 100ug
