PRDX5 (peroxiredoxin 5) also known as AOEB166, ACR1, B166, PLP, PMP20, PRDX6, PRXV, SBBI10, is a member of the peroxiredoxin family and may play an antioxidant protective role in various tissues under nonpathologic conditions and during inflammatory processes.
The PRDX5 gene is mapped to11q13.1.
PRDX5 displays mitochondrial presequence features and has 3 cysteines implicated in antioxidant activity and a C-terminal SQL peroxisomal targeting sequence.
Northern blot analysis revealed ubiquitous expression of a 1.0-kb PRDX5 transcript in tissues and cell lines.
Functional analysis showed that PRDX5 has antioxidant activity equivalent to that of CAT.
While PRDX5 was localized to fibroblasts in normal tendon, it was localized to fibroblasts and endothelial cells in degenerative tendon.
PRDX5 mRNA and protein levels increased at 12 hours, and the increase in PRDX5 expression correlated with reduced peroxide levels.
PRDX5 plays a protective role against oxidative stress in human cartilage.
Applications:Suitable for use in Western Blot, Immunohistochemistry and Immunocytochemistry.
Other applications not tested.
Recommended Dilutions:Western Blot: 0.1-0.5ug/ml, the detection limit is ~0.1ng/lane under reducing conditions
Immunohistochemistry (Paraffin): 0.5-1ug/ml.
Boiling the paraffin sections in 10mM citrate buffer, pH 6.0, for 20 minutess is required for the staining of formalin/paraffin sections
Immunocytochemistry: 0.5-1ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C.
Reconstitute with sterile ddH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from PBS, 5% BSA, 0.05% sodium azide. Reconstitute with 200ul sterile dH2O.
Specificity：Recognizes human Peroxiredoxin 5. Species Crossreactivity: mouse and rat.
Isotype：IgG
Calc Applications Abbrev：IC IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Recombinant protein corresponding to human Peroxiredoxin 5 expressed in E. coli. (Position: E66-D198). Species Sequence Homology: mouse and rat, 91%.
