PRDX1(Peroxiredoxin 1), also called PRX1, PAGA or NKEFA, is a thiol reductase that plays critical roles in oxidative and thermal stress defense mechanisms through its abilities to metabolize H2O2 and act as a molecular chaperone, respectively.
This gene encodes a member of the peroxiredoxin family of antioxidant enzymes, which reduce hydrogen peroxide and alkyl hydroperoxides.
The PRDX1 gene is mapped on 1p34.1.
Prdx1 was expressed in differentiating motor neuron cells in developing embryonic chicken and mouse spinal cords.
Immunoprecipitation analysis showed that GDE2 interacted directly with PRDX1 in embryonic chicken spinal cord extracts and in transfected HEK293T cells.
This protein may have a proliferative effect and play a role in cancer development or progression.
In differentiating spinal cord, Prdx1 was required to activate Gde2 by reducing an intramolecular cystine bridge between the Gde2 N- and C-terminal domains.
An intramolecular disulfide bond between the GDE2 N- and C-terminal domains inhibits GDE2 function, and that reduction of this cystine by PRDX1 activates GDE2 for the induction of motor neuron differentiation.
Applications:Suitable for use in Western Blot, Immunohistochemistry and Immunocytochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 0.1-0.5ug/ml, the detection limit is ~0.1ng/lane under reducing conditions
Immunohistochemistry (paraffin): 0.5-1ug/ml, boiling the paraffin sections in 10mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections
Immunocytochemistry: 0.5-1ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months after receipt at -20°C.
Reconstitute with sterile ddH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from PBS, 5% BSA, 0.05% sodium azide. Reconstitute with 200ul sterile dH2O.
Specificity：Recognizes human Peroxiredoxin 1. Species Crossreactivity: mouse and rat
Isotype：IgG
Calc Applications Abbrev：IC IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to aa100-128, MNIPLVSDPKRTIAQDYGVLKADEGISFR, from the middle region of human Peroxiredoxin 1, different from the related mouse sequence by 1aa, and identical to the related rat sequence.
Shelf Life：1year
Swiss Prot Number：Q06830
EU Commodity Code：30021010
商品名：Peroxiredoxin 1 (Hbp23, Heme Binding 23kD Protein, Macrophage 23kD Stress Protein, Macrophase Stress Protein 22kD, Macrophase Stress Protein 23kd, MSP23, Natural Killer Cell Enhancing Factor A, Natural Killer Cell-enhancing Factor A, Natural Killer Enhancing Factor A, NKEF A, NKEF-A, NkefA, OSF3, Osteoblast Specific Factor 3, PAG, Paga, PAGB, Peroxiredoxin-1, Prdx 1, Prdx1, PRDX1_HUMAN, Proliferation Associated Gene A, Proliferation Associated Protein PAG, Proliferation-associated Gene Protein, PrxI, Tdpx 2, Tdpx2, TDX2, Thioredoxin Dependent Peroxide Reductase 2, Thioredoxin Peroxidase 2, Thioredoxin-dependent Peroxide Reductase 2, TPxA, Trx Dependent Peroxide Reductase 2) 100ug
