Tumor protein p63 (TP63), also known as the p63 or TP73L is a protein that in humans is encoded by the TP63 gene.
It is mapped to 3q28.
TP63 is a member of the p53 family of transcription factors.
This gene encodes for two main isoforms by alternative promoters (TAp63 and DNp63).
TP63 has been mostly restricted to its apoptotic function and more recently as the guardian of oocyte integrity.
It has been found that the combined loss of TP63 and p73 results in the failure of cells containing functional p53 to undergo apoptosis in response to DNA damage.
TP63 is an essential regulator of stem cell maintenance in stratified epithelial tissues.
Tp63 is also critical for maintaining the progenitor-cell populations that are necessary to sustain epithelial development and morphogenesis.
Applications:Suitable for use in Western Blot, Immunohistochemistry and Immunocytochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 0.1-0.5ug/ml.
The detection limit for p63 is ~0.25ng/lane under reducing conditionsImmunohistochemistry (paraffin): 0.5-1ug/ml.
Boiling the paraffin sections in 10mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections
Immunocytochemistry: 0.5-1ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C.
Reconstitute with sterile ddH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from PBS, 5% BSA, 0.05% sodium azide. Reconstitute with 200ul sterile ddH2O.
Specificity：Recognizes human p63.
Isotype：IgG
Calc Applications Abbrev：IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to human p63 expressed in E.coli. (Position: S311-E680). Species sequence homology: mouse and rat (98%)
Shelf Life：1year
Swiss Prot Number：Q9H3D4
EU Commodity Code：30021010
商品名：p63 (AIS, Amplified in Squamous Cell Carcinoma, B(p51A), B(p51B), Chronic Ulcerative Stomatitis Protein, CUSP, DN p63 alpha 1, DNp63, EEC3, Keratinocyte Transcription Factor, Keratinocyte Transcription Factor KET, KET, LMS, NBP, OFC8, p40, p51, P51/P63, p53 Like Transcription Factor, p53 Related Protein, p53-related Protein p63, p53CP, P63_HUMAN, P73, p73H, p73L, RHS, SHFM4, TAp63alpha, TP53CP, TP53L, TP63, TP73, TP73L, Transformation Related Protein 63, Transformation-related Protein 63, Trp53rp1, Trp63, Tumor Protein 63, Tumor Protein 63kD With Strong Homology to p53, Tumor Protein p53-competing Protein, Tumor Protein p53-like, Tumor Protein p63, Tumor Protein p63 deltaN Isoform delta, Tumor Protein p73, Tumor Protein p73-like) 100ug
