Nuclear pore complex protein Nup98-Nup96 is a protein that in humans is encoded by the NUP98 gene.
This gene is one of several genes located in the imprinted gene domain of 11p15.5, an important tumor-suppressor gene region.
NUP98 is a peripheral nucleoporin located at both the cytoplasmic and nuclear sides of the central channel of the NPC.
NUP98 phosphorylation is critical for NPC disassembly at the onset of mitosis.
It also plays roles in gene expression, mitotic checkpoint, and pathogenesis.
Ligand blot analysis suggested that NUP98 can function as a docking protein for cytosol-mediated docking of import substrates.
In addition to that, NUP98 is a target of the vesicular stomatitis virus M protein-mediated inhibition of mRNA nuclear export.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 0.1-0.5ug/ml, the detection limit is ~0.1ng/lane under reducing conditions
Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months after receipt at -20°C.
Reconstitute with sterile ddH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from PBS, 5% BSA, 0.05% sodium azide. Reconstitute with 200ul sterile dH2O.
Specificity：Recognizes human NUP98. Species Crossreactivity: mouse and rat
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Recombinant protein corresponding to human NUP98 expressed in E. coli. (Position: H549-F880). Species sequence homology: mouse (95%) and rat (94%)
