84-3433-98　Nucleophosmin (B23, NMP1, NO38, NPM 1, NPM, NPM_HUMAN, NPM1, Nucleolar Phosphoprotein B23, Nucleolar Protein NO38, Nucleophosmin (Nucleolar Phosphoprotein B23 Numatrin), Nucleophosmin Nucleoplasmin Family Member 1, Nucleophosmin/B23.2, Nucleophosmin/Nucleoplasmin Family Member 1, Nucleoplasmin Family Member 1, Numatrin, TRK Fused Gene) 100ug　303494

特徴

• NPM1(Nucleophosmin/Nucleoplasmin family, member1), also known as NPM, nucleolar phosphoprotein B23 or numatrin, is a protein that in humans is encoded by the NPM1 gene.
• The NPM1 gene maps to chromosome 5q35.
• Chan et al.
• (1989) found that nucleophosmin is a nucleolar phosphoprotein that is more abundant in tumor cells than in normal resting cells.
• Stimulation of the growth of normal cells, e.g., mitogen activation of B lymphocytes, was accompanied by an increase in nucleophosmin protein level.
• They stated that nucleophosmin is likely involved in the assembly of ribosomal proteins into ribosomes.
• Electron microscopic study indicated that nucleophosmin is concentrated in the granular region of the nucleolus, where ribosome assembly occurs.
• Applications:Suitable for use in Western Blot and Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:Western Blot: 0.1-0.5ug/ml, the detection limit is ~0.1ng/lane under reducing conditions
• Immunohistochemistry (paraffin): 0.5-1ug/ml, boiling the paraffin sections in 10mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections
• Optimal dilutions to be determined by the researcher.
• Storage and Stability:Lyophilized powder may be stored at -20°C.
• Stable for 12 months after receipt at -20°C.
• Reconstitute with sterile ddH2O.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Reconstituted product is stable for 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.