84-3433-89　NFkB, p100/p52 (DNA Binding Factor KBF2, DNA-binding Factor KBF2, H2TF1, Lymphocyte Translocation Chromosome 10, Lymphocyte Translocation Chromosome 10 Protein, Lyt 10, Lyt10, NF kB2, NFKB2, NFKB2_HUMAN, Nuclear Factor NF kappa B p100 Subunit, Nuclear Factor NF kappa B p52 Subunit, Nuclear Factor NF-kappa-B p52 Subunit, Nuclear Factor of kappa Light Chain Gene Enhancer in B Cells 2, Nuclear Fa ...　303482

特徴

• NFKB2, also known as nuclear factor NF-kappa-B p100 subunit, is a protein that in humans is encoded by the NFKB2 gene.
• It is mapped to 10q24.32.
• This gene encodes a subunit of the transcription factor complex nuclear factor-kappa-B (NFKB).
• NFKB is activated by a wide variety of stimuli such as cytokines, oxidant-free radicals, inhaled particles, ultraviolet irradiation, and bacterial or viral products.
• The NFkB complex is expressed in numerous cell types and functions as a central activator of genes involved in inflammation and immune function.
• The protein encoded by this gene can function as both a transcriptional activator or repressor depending on its dimerization partner.
• Applications:Suitable for use in Western Blot and Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:Western Blot: 0.1-0.5ug/ml, the detection limit is ~0.25ng/lane under reducing conditions
• Immunohistochemistry (paraffin): 0.5-1ug/ml, boiling the paraffin sections in 10mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections
• Optimal dilutions to be determined by the researcher.
• Storage and Stability:Lyophilized powder may be stored at -20°C.
• Stable for 12 months after receipt at -20°C.
• Reconstitute with sterile ddH2O.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Reconstituted product is stable for 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.