84-3433-88　NFkB p105/P50 (DNA Binding Factor KBF1, DNA Binding Factor KBF1 EBP1, DNA-binding Factor KBF1, EBP 1, EBP-1, EBP1, KBF1, NF kappa B, NF kappaB, NF kappabeta, NF kB1, NFkappaB, NFKB 1, NFKB p105, NFKB p50, NFKB1, NFKB1_HUMAN, Nuclear Factor kappa B DNA Binding Subunit, Nuclear Factor kappa-B, Subunit 1, Nuclear Factor NF kappa B p105 Subunit, Nuclear Factor NF kappa B p50 Subunit, Nuclear Facto ...　303481

特徴

• Nuclear factor NF-kappa-B p105 subunit, also called EBP-1, is a protein that in humans is encoded by the NFKB1 gene.
• By fluorescence in situ hybridization, the gene was assigned to human chromosome 4q24.
• NFKB1 is a pleiotropic transcription factor present in almost all cell types and is the endpoint of a series of signal transduction events that are initiated by a vast array of stimuli related to many biological processes such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis.
• NFKB1 appears to have dual functions such as cytoplasmic retention of attached NFKB1 proteins by p105 and generation of p50 by a cotranslational processing.
• Applications:Suitable for use in Western Blot and Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:Western Blot: 0.1-0.5ug/ml, the detection limit is ~0.25ng/lane under reducing conditions
• Immunohistochemistry (paraffin): 0.5-1ug/ml, boiling the paraffin sections in 10mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections
• Optimal dilutions to be determined by the researcher.
• Storage and Stability:Lyophilized powder may be stored at -20°C.
• Stable for 12 months after receipt at -20°C.
• Reconstitute with sterile ddH2O.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Reconstituted product is stable for 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.