Mucin 1, cell surface associated (MUC1) or polymorphic epithelial mucin (PEM) is a mucin encoded by the MUC1 gene in humans.
This gene encodes a membrane-bound protein that is a member of the mucin family.
Mucins are O-glycosylated proteins that play an essential role in forming protective mucous barriers on epithelial surfaces.
It is mapped to 1q22.
Mucin 1 is a transmembrane mucin normally expressed on the apical borders of secretory epithelial cells.
Overexpression of Mucin 1 is often associated with colon, breast, ovarian, lung and pancreatic cancers.
The protein serves a protective function by binding to pathogens and also functions in a cell signaling capacity.
Mucin 1 stimulated ESR1-mediated transcription and contributed to estradiol-mediated growth and survival of breast cancer cells.
This gene also can suppress pulmonary innate immunity, and its anti-inflammatory activity may play an important modulatory role during microbial infection.
Applications:Suitable for use in Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 0.1-0.5ug/ml, the detection limit is ~0.25ng/lane under reducing conditions
Immunohistochemistry (frozen): 0.5-1ug/mlImmunohistochemistry (paraffin): 0.5-1ug/ml, boiling the paraffin sections in 10mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections
Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months after receipt at -20°C.
Reconstitute with sterile ddH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from PBS, 5% BSA, 0.05% sodium azide. Reconstitute with 200ul sterile dH2O.
Specificity：Recognizes mouse MUC1. Species Crossreactivity: rat
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Mo Rt
Immunogen：Recombinant protein corresponding to mouse MUC1 expressed in E. coli. (Position: S474-L630). Species sequence homology: human (87%)
