Matrix metalloproteinase-7 (MMP-7) previously called putative metalloproteinase I (PUMP1) or matrilysin.
The MMP-7 gene has been identified through studies of collagenase-related connective-tissue-degrading metalloproteinases produced by human tumors.
The MMP-7 protein has 267aa and is significantly shorter than stromelysin or collagenase (477aa and 469aa, respectively).
Matrix metalloproteinases play a crucial role in tumor invasion and metastasis.
MMP-7, a member of the matrix metalloproteinase family, is structurally different from the other matrix metalloproteinases by virtue of the absence of a conserved COOH-terminal protein domain.
In addition, MMP-7 mRNA is regulated in a specific and distinct manner in normal and malignant tissues.
MMP-7 has been shown to correlate with nodal or distant metastasis in colorectal carcinomas, however, its implication in early invasive colorectal carcinomas has not been determined.
MMP-7 is also a mediator of pulmonary fibrosis and a potential therapeutic target.
The standard product used in this kit is recombinant human MMP-7, consisting of 250aa with the molecular mass of 28kD.
The detected MMP-7 includes zymogen and active enzyme.
Applications:Suitable for use in ELISA, Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 0.1-0.5ug/mlWestern Blot: 0.1-0.5ug/ml, the detection limit is ~0.25ng/lane under reducing conditions
Immunohistochemistry (paraffin): 0.5-1ug/ml, boiling the paraffin sections in 10mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections
Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months after receipt at -20°C.
Reconstitute with sterile ddH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from PBS, 5% BSA, 0.05% sodium azide. Reconstitute with 200ul sterile dH2O.
Specificity：Recognizes human MMP7.
Isotype：IgG
Calc Applications Abbrev：E IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to human MMP7 expressed in E. coli. (Position: M1-K267). Species sequence homology: mouse and rat (71%)
