Mitofusin-1 is a protein that in humans is encoded by the MFN1 gene.
It is an 8090kD mitochondrial member of the dynamin family of molecules.
It is ubiquitously expressed, and found in the outer mitochondrial membrane.
The protein encoded by this gene is a mediator of mitochondrial fusion, and thereby contribute to the dynamic balance between fusion and fission that determines mitochondria morphology.
MFN1 is known to form oligomers, either with itself or MFN2, and to undergo ubiquitination by MARCH5.
MFN1 has two key domains.
One is a coiledcoil region that mediates MFN1: MFN1/2 binding, and a second is a GTPase domain whose cleavage of GTP is necessary for membrane fusion.
Overexpression of MFN1 caused perinuclear mitochondrial clustering.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilutions:Western Blot: 0.1-0.5ug/ml, the detection limit is ~0.25ng/lane under reducing conditions
Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C.
Reconstitute with sterile dH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography
Form：Supplied as a lyophilized powder from PBS, 5% BSA, 0.05% sodium azide. Reconstitute with 200ul sterile dH2O.
Specificity：Recognizes human Mitofusin 1. Species Crossreactivity: mouse and rat
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to aa23-49, DQLLEFVTEGSHFVEATYKNPELDRIA at the N-terminal of human Mitofusin 1. Sequence is different from the related mouse and rat sequences by one amino acid
