The MEN1 gene encodes menin, a nuclear scaffold protein that regulates gene transcription by coordinating chromatin remodeling.
It is mapped to 11q13.1.
MEN1 is considered to act as a tumor suppressor gene.
It has been found that that MEN1 inactivation by antisense RNA antagonizes transforming growth factor-beta-mediated cell growth inhibition.
Overexpression of MEN1 in an inducible cell culture system downregulated the proximal promoter.
In vitro studies have shown that MEN1 is localized to the nucleus, possesses two functional nuclear localization signals, and inhibits transcriptional activation by JunD.
MEN1 was essential to maintain MLL-associated myeloid transformation.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 0.1-0.5ug/ml, the detection limit is ~0.25ng/lane under reducing conditions
Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months after receipt at -20°C.
Reconstitute with sterile ddH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from PBS, 5% BSA, 0.05% sodium azide. Reconstitute with 200ul sterile dH2O.
Specificity：Recognizes human Menin. Species Crossreactivity: mouse
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo
Immunogen：Recombinant protein corresponding to human Menin expressed in E. coli. (Position: P301-L615). Species sequence homology: mouse (93%) and rat (94%)
