Methyl-CpG-binding domain protein 2 is a protein that in humans is encoded by the MBD2 gene.
It is mapped to 18q21.2.
Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG-binding domain (MBD).
Each of these proteins, with the exception of MBD3, is capable of binding specifically to methylated DNA.
DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development.
The protein encoded by this gene may function as a mediator of the biological consequences of the methylation signal.
It is also reported that this protein functions as a demethylase to activate transcription, as DNA methylation causes gene silencing.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 0.1-0.5ug/ml, the detection limit is ~0.2ng/lane under reducing conditions
Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile dH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from PBS, 5% BSA, 0.05% sodium azide. Reconstitute with 200ul sterile dH2O.
Specificity：Recognizes human MBD2.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to human MBD2 expressed in E. coli. (Position: W159-A411). Species sequence homology: mouse (98%)
