Mitogen-activated protein kinase 1, also known as MAPK1, p42MAPK, and ERK2, is an enzyme which in humans is encoded by the MAPK1 gene.
The gene is mapped to 22q11.2.
The protein encoded by this gene is a member of the MAP kinase family.
The deduced 360aa human ERK2 protein shares 98% identity with rat Erk2.
The MAP kinase ERK2 is widely involved in eukaryotic signal transduction.
Upon activation, it translocates to the nucleus of the stimulated cell, where it phosphorylates nuclear targets.
Futhermore, the ERK pathway is necessary for experience- dependent plasticity and for long-term potentiation of synaptic transmission in the developing visual cortex.
And the MAPK pathway is also involved in responses to NTN1.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 0.1-0.5ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile dH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from PBS, 5% BSA, 0.05% thimerosal, 0.05% sodium azide. Reconstitute with 200ul sterile dH2O.
Specificity：Recognizes human MAPK2. Species Crossreactivity: mouse and rat
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to aa324-335, IAEAPFKFDMEL, from human MAPK2 at C-terminus, identical to the related rat and mouse sequences.
