84-3433-43　LIF (CDF, Cholinergic Differentiation Factor, D Factor, DIA, Differentiation Inducing Factor, Differentiation Inhibitory Activity, Differentiation Stimulating Factor, Differentiation-stimulating Factor, Emfilermin, Hepatocyte Stimulating Factor III, HILDA, Human Interleukin in DA Cells, Leukemia Inhibitory Factor, LIF_HUMAN, Melanoma Derived LPL Inhibitor, Melanoma-derived LPL Inhibitor, MLPLI ...　303428

特徴

• LIF is a pleiotropic cytokine produced at the maternal-fetal interface which has been shown to play an essential role in implantation in mice.
• This gene is mapped to 22q11-q12.2, between the Philadelphia translocation BCR gene and the breakpoint of the translocation in cell line GM2324 at 22q12.2.
• LIF is produced in high amounts by the human endometrium and the trophoblast itself, and LIF receptors are present on cytotrophoblast cells.
• LIF could, thus, play a role in modulating HLA-G production and immune tolerance at the maternal-fetal interface.
• Applications:Suitable for use in Western Blot and Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:Western Blot: 0.1-0.5ug/ml, the detection limit is ~0.25ng/lane under reducing conditions
• Immunohistochemistry (paraffin): 0.5-1ug/ml, boiling the paraffin sections in 10mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections
• Optimal dilutions to be determined by the researcher.
• Storage and Stability:Lyophilized powder may be stored at -20°C.
• Stable for 12 months after receipt at -20°C.
• Reconstitute with sterile ddH2O.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Reconstituted product is stable for 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.