84-3433-29　JunB, CT (Activator Protein 1, AP 1, AP1, Jun B, Jun B Proto Oncogene, Jun B Protooncogene, Junb, JunB Proto Oncogene, JunB Protoncogene 9, JunB Protooncogene, JUNB_HUMAN, Transcription Factor Jun B, Transcription Factor Jun-B, Transcription Factor JunB) 100ug　303409

特徴

• Transcription factor jun-B (JUNB) is a protein that in humans is encoded by the JUNB gene.
• It is mapped to 19p13.2.
• JUNB is a transcription factor involved in regulating gene activity following the primary growth factor response.
• It binds to the DNA sequence 5'-TGA[CG]TCA-3', and a large fraction (over 50%) of the JUNB locus is contained in these flanking evolutionarily conserved sequences (FECS), which may be required for effecting the proper transcriptional regulation of this gene.
• The expression of JUNB gene might be involved in terminal granulocyte differentiation or in regulating granulocyte functionality.
• Applications:Suitable for use in Western Blot and Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:Western Blot: 0.1-0.5ug/ml, the detection limit is ~0.25ng/lane under reducing conditions
• Immunohistochemistry (paraffin): 0.5-1ug/ml, boiling the paraffin sections in 10mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections
• Optimal dilutions to be determined by the researcher.
• Storage and Stability:Lyophilized powder may be stored at -20°C.
• Stable for 12 months after receipt at -20°C.
• Reconstitute with sterile ddH2O.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Reconstituted product is stable for 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.