Inositol 1,4,5-trisphosphate receptor type 1, also known as IP3R or IP3R1, is a protein that in humans is encoded by the ITPR1 gene.
It is mapped to 3p26.1.
The product of the ITPR1 gene is predominantly enriched in cerebellar Purkinje cells but is also concentrated in neurons in the hippocampal CA1 region, caudate-putamen, and cerebral cortex.
The ITPR1 gene encodes the inositol 1,4,5-triphosphate (IP3) receptor, an intracellular IP3-gated calcium channel that modulates intracellular calcium signaling.
Upon stimulation by inositol 1,4,5-trisphosphate, this receptor mediates calcium release from the endoplasmic reticulum.
Mutations in ITPR1 cause spinocerebellar ataxia type 15, a disease associated with an heterogeneous group of cerebellar disorders.
Applications:Suitable for use in Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 0.1-0.5ug/ml, the detection limit is ~0.2ng/lane under reducing conditions
Immunohistochemistry (paraffin): 0.5-1ug/ml, boiling the paraffin sections in 10mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections
Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months after receipt at -20°C.
Reconstitute with sterile ddH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from PBS, 5% BSA, 0.05% sodium azide. Reconstitute with 200ul sterile dH2O.
Specificity：Recognizes human IP3 receptor. Species Crossreactivity: mouse and rat
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Recombinant protein corresponding to human IP3 receptor expressed in E. coli. (Position: A2411-A2758). Species sequence homology: mouse (98%) and rat (97%)
Shelf Life：1year
Swiss Prot Number：Q14643
EU Commodity Code：30021010
商品名：ITPR1 (IP3 Receptor, 5-trisphosphate Receptor, 5-trisphosphate Receptor Type 1, Inositol 1 4 5 Triphosphate Receptor Type 1, Inositol 1 4 5 Trisphosphate Receptor Type 1, Inositol 1, Inositol 145-trisphosphate Receptor Type 1, InsP3R1, IP3, IP3 Receptor Isoform 1, IP3R 1, IP3R, IP3R1, ITPR 1, Itpr1, ITPR1_HUMAN, SCA15, SCA16, SCA29, Type 1 Inositol 1 4 5 Trisphosphate Receptor, Type 1 Inositol 1, Type 1 InsP3 Receptor) 100ug
