Integrin beta-1, also known as CD29, is a protein that in humans is encoded by the ITGB1 gene.
CD29 is an integrin unit associated with very late antigen receptors.
It is known to conjoin with alpha-3 subunit to create alpha3beta1 complex that reacts to such molecules as netrin-1 and reelin.
This gene contains a beta subunit that appears to be analogous to band-3 of integrin.
It is mapped to 10p11.22.
It is found that FER mediates crosstalk between CDH2 and CD29.
Integrin family members are membrane receptors involved in cell adhesion and recognition in a variety of processes including embryogenesis, hemostasis, tissue repair, immune response and metastatic diffusion of tumor cells.
The protein encoded by this gene is a beta subunit.
Applications:Suitable for use in Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 0.1-0.5ug/ml, the detection limit is ~0.25ng/lane under reducing conditions
Immunohistochemistry (paraffin): 0.5-1ug/ml, boiling the paraffin sections in 10mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections
Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months after receipt at -20°C.
Reconstitute with sterile ddH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from PBS, 5% BSA, 0.05% sodium azide. Reconstitute with 200ul sterile dH2O.
Specificity：Recognizes human ITGB1.
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to human ITGB1 expressed in E. coli. (Position: N527-D728). Species sequence homology: mouse (91%) and rat (88%)
Shelf Life：1year
Swiss Prot Number：P05556
EU Commodity Code：30021010
商品名：ITGB1 (Beta 1 Subunit, CD 29, CD29, CD29 Antigen, Fibrinogen Receptor beta Subunit, Fibronectin Receptor Subunit beta, FNRB, Glycoprotein IIa, GP IIa, GPIIA, Integrin beta 1 (Fibronectin Receptor beta Polypeptide Antigen CD29 Includes MDF2 MSK12), Integrin beta-1, Integrin VLA 4 Subunit beta, Integrin VLA-4 beta Subunit, Integrin, beta 1 (Fibronectin Receptor, beta Polypeptide, Antigen CD29 Includes MDF2, MSK12), ITGB 1, MDF2, MSK12, Very Late Activation Protein beta Polypeptide, Very Late Activation Protein, beta Polypeptide, VLA Beta, VLA-4 Subunit beta, VLA-Beta, VLAB, VLAbeta) 100ug
