Interferon regulatory factor 1, also known as MAR is a protein that in humans is encoded by the IRF1 gene.
The IRF1 gene is mapped to chromosome 5q31.1 by pulsed-field gel electrophoresis.
IRF1 encodes interferon regulatory factor 1, a member of the interferon regulatory transcription factor(IRF) family.
IRF1 serves as an activator of interferons alpha and beta transcription, and in mouse it has been shown to be required for double-stranded RNA induction of these genes.
IRF1 also functions as a transcription activator of genes induced by interferons alpha, beta, and gamma.
Further, IRF1 has been shown to play roles in regulating apoptosis and tumor-suppressoion.
Applications:Suitable for use in Western Blot, Immunohistochemistry and Immunocytochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 0.1-0.5ug/mlImmunohistochemistry (paraffin): 0.5-1ug/ml, boiling the paraffin sections in 10mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections
Immunocytochemistry: 0.5-1ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile dH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from PBS, 5% BSA, 0.05% thimerosal, 0.05% sodium azide. Reconstitute with 200ul sterile dH2O.
Specificity：Recognizes human IRF1.
Isotype：IgG
Calc Applications Abbrev：IC IHC WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to aa169-183, YMQDLEVEQALTPAL, from the middle region of human IRF1.
