84-3433-12　IL6 (Interleukin BSF 2, B Cell Differentiation Factor, B Cell Stimulatory Factor 2, B-cell Stimulatory Factor 2, BSF 2, BSF-2, BSF2, CDF, CTL Differentiation Factor, Cytotoxic T Cell Differentiation Factor, Hepatocyte Stimulating Factor, HGF, HPGF, HSF, Hybridoma Growth Factor, Hybridoma Growth Factor Interferon beta-2, Hybridoma Plasmacytoma Growth Factor, IFN-beta-2, IFNB2, IL 6, IL-6, IL6 P ...　303390

特徴

• Interleukin-6 (IL-6) is a protein that in humans is encoded by the IL6 gene.
• IL-6 is an interleukin that acts as both a pro-inflammatory and anti-inflammatory cytokine.
• It is secreted by T cells and macrophages to stimulate immune response to trauma, especially burns or other tissue damage leading to inflammation.
• IL-6 is one of the most important mediators of fever and of the acute phase response.
• IL-6 is also essential for hybridoma growth and is found in many supplemental cloning media such as briclone.
• Bowcock et al.
• (1988) assigned the IL6 gene to chromosome 7p21.
• By in situ hybridization and Southern blot analysis of mouse-human hybrid cell lines, Sutherland et al.
• (1988) mapped the IL-6 gene to chromosome 7p15.
• Applications:Suitable for use in Western Blot.
• Other applications not tested.
• Recommended Dilution:Western Blot: 0.1-0.5ug/ml, the detection limit is ~5ng/lane under reducing conditions
• Optimal dilutions to be determined by the researcher.
• Storage and Stability:Lyophilized powder may be stored at -20°C.
• Stable for 12 months after receipt at -20°C.
• Reconstitute with sterile ddH2O.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Reconstituted product is stable for 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.