Interleukin 3, also known as IL-3, is a protein that in humans is encoded by the IL3 gene.
It is mapped to 5q31.1.
IL-3 is an interleukin, a type of biological signal (cytokine) that can improve the body's natural response to disease as part of the immune system.
It acts by binding to the interleukin-3 receptor.
IL-3 stimulates the differentiation of multipotent hematopoietic stem cells into myeloid progenitor cells or, with the addition of IL-7, into lymphoid progenitor cells.
In addition, IL-3 stimulates proliferation of all cells in the myeloid lineage (granulocytes, monocytes, and dendritic cells), in conjunction with other cytokines, e.g., Erythropoietin (EPO), Granulocyte macrophage colony-stimulating factor (GM-CSF), and IL-6.
IL-3 is secreted by basophils and activated T cells to support growth and differentiation of T cells from the bone marrow in an immune response.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:ELISA: 0.1-0.5ug/mlWestern Blot: 0.1-0.5ug/ml, the detection limit is ~0.25ng/lane under reducing conditions
Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile dH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：rat
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from PBS, 5% BSA, 0.05% sodium azide. Reconstitute with 200ul sterile dH2O.
Specificity：Recognizes rat IL3.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Rt
Immunogen：Recombinant protein corresponding to rat IL3 expressed in E. coli. (Position: S28-C166). Species sequence homology: human (32%) and mouse (55%)
