Interleukin-2 (IL2), formerly referred to as T-cell growth factor, is a powerfully immunoregulatory lymphokine that is produced by lectin- or antigen-activated T cells.
It is produced not only by mature T lymphocytes on stimulation but also constitutively by certain T-cell lymphoma cell lines.
The lymphokine interleukin-2 (IL-2) is responsible for autocrine cell cycle progression and regulation of immune responses.
IL-2 expression in mature thymocytes and T cells has been found to be tightly controlled by monoallelic expression.
IL-2 can act as a growth hormone for both B and T lymphocytes.
This gene for interleukin 2 (IL2) is assigned to chromosome 4.
IL-2 is a 15.5kD glycoprotein, consisting of 153aa in precursor form and 133aa in mature form.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 0.1-0.5ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months after receipt at -20°C.
Reconstitute with sterile ddH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size:100ug
Host:rabbit
Source Antibody:rat
Grade:Affinity Purified
Purity:Purified by immunoaffinity chromatography.
Form:Supplied as a lyophilized powder from PBS, 5% BSA, 0.05% sodium azide. Reconstitute with 200ul sterile dH2O.
Specificity:Recognizes rat IL2.
Isotype:IgG
Calc Applications Abbrev:WB
Calc Crossreactivity:Rt
Immunogen:Recombinant protein corresponding to rat IL2 expressed in E. coli. (Position: A21-Q155). Species sequence homology: human (66%) and mouse (80%)