Subunit beta of interleukin 12, also known as interleukin 12B, or IL12B, is a subunit of human interleukin 12.
It is mapped to 5q33.3.
This cytokine is expressed by activated macrophages that serve as an essential inducer of Th1 cells development.
This cytokine has been found to be important for sustaining a sufficient number of memory/effector Th1 cells to mediate long-term protection to an intracellular pathogen.
Overexpression of this gene was observed in the central nervous system of patients with multiple sclerosis (MS), suggesting a role of this cytokine in the pathogenesis of the disease.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 0.1-0.5ug/ml, the detection limit is ~0.25ng/lane under reducing conditions
Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months at -20°C.
Reconstitute with sterile dH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：rat
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder in 0.2mg Na2HPO4, 5mg BSA, 0.9mg sodium chloride, 0.05mg sodium azide. Reconstitute with 200ul sterile dH2O to 500ug/ml.
Specificity：Recognizes rat IL12B.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Rt
Immunogen：Recombinant protein corresponding to rat IL12B expressed in E. coli. (Position: M23-S321).
