NF-kappa-B inhibitor beta, also known as IKBB or TRIP9, is a protein that in humans is encoded by the NFKBIB gene.
The protein encoded by this gene belongs to the NF-kappa-B inhibitor family, which inhibit NF-kappa-B by complexing with, and trapping it in the cytoplasm.
This gene is mapped to 19q13.2.
It has been found that in vivo, NFKBIB serves both to inhibit and to facilitate the inflammatory response.
NFKBIB degradation releases NF-kappa-B dimers, which upregulate proinflammatory target genes such as TNF-alpha.
Surprisingly, absence of NFKBIB results in a dramatic reduction of TNF-alpha in response to lipopolysaccharide, even though activation of NF-kappa-B is normal.
Applications:Suitable for use in Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 0.1-0.5ug/ml, the detection limit is ~0.25ng/lane under reducing conditions
Immunohistochemistry (paraffin): 0.5-1ug/ml, boiling the paraffin sections in 10mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections
Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months after receipt at -20°C.
Reconstitute with sterile ddH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a lyophilized powder from PBS, 5% BSA, 0.05% sodium azide. Reconstitute with 200ul sterile dH2O.
Specificity：Recognizes human IKB beta. Species Crossreactivity: mouse and rat
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Recombinant protein corresponding to human IKB beta expressed in E. coli. (Position: E56-E237). Species sequence homology: mouse (82%) and rat (80%)
